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  BH News 2016-07 New SPCM Software Runs Online-FLIM at a Rate of 10 Images per Second
¤ýÀÛ¼ºÀÚ: ÀüÀçÇÊ ¤ýÀÛ¼ºÀÏ: 2016-07-28 (¸ñ) 16:46 ¤ýÁ¶È¸: 692

BH News 2016-07

New SPCM Software Runs Online-FLIM at a Rate of 10 Images per Second

With version 9.72 64-bit SPCM software, bh FLIM systems record and display fluorescence lifetime images at a rate of up to 10 images per second. The data are recorded by bh¡¯s multi-dimensional TCSPC technique in combination with confocal or multiphoton laser scanning, the images are created by first-moment analysis. The technique combines near-ideal photon efficiency with short acquisition and calculation times. It works for all SPC-150, SPC-150N, SPC-160, and SPC-830 FLIM systems that use fast scanning. The figure below shows online-FLIM images recorded with 128x128 pixels at a rate of 10 images per second (left) and with 256x256 pixels at a rate of 2 images per second (right).

For details, please see application note under www.becker-hickl.com/pdf/online-flim-06.pdf.


FLIM of Convallaria sample. Left: 128x128 pixels, 10 images per second. Right: 256x256 pixels, 2 image per second. Lifetime range 1ns (red) to 3ns (blue).
 

Please click [here] for full application note.