ÀÚ·á½Ç

 

ÀÚ·á½Ç

 

 

search

 

 

search

 

 

 

 

 

¤ýHOME > ÀÚ·á½Ç

 

  BH News 2016-11 bh TCSPC Systems Record FLIM with Sutter MOM Microscopes
¤ýÀÛ¼ºÀÚ: ÀüÀçÇÊ ¤ýÀÛ¼ºÀÏ: 2016-11-22 (È­) 10:46 ¤ýÁ¶È¸: 501

BH News 2016-11

bh TCSPC Systems Record FLIM with Sutter MOM Microscopes

The Sutter Instrument MOM microscope is a modular platform for fluorerscence imaging deep within live samples. It uses multi-photon excitation by a titanium-sapphire laser in combination with non-descanned detection. Due to its pulsed excitation source and its high modularity the MOM system can easily be combined with the bh TCSPC FLIM systems. Up to four FLIM detectors can be attached to the system. The signals are processed in up to four entirely parallel TCSPC FLIM channels. Due to the parallel system architecture, high photon count rates and short acquisition times can be achieved. Multiphoton excitation and non-descanned detection make the system especially useful for FLIM of live cells and tissues. FLIM data can be recorded with up to 1024x1024 pixels and 1024 time channels per pixel. Typical applications are metabolic imaging by recording the fluorescence of NADH and FAD, protein interaction experiments by FLIM-FRET techniques, and ion concentration measurements with environment-sensitive fluorescent dyes.

           

Left: Sutter MOM with two FLIM detectors. Right: FLIM of Lepeophtheirus Salmonis, two-photon excitation at 750 nm, detection at 460 nm



(Click here for application note)