•    Record a time-series mosaic of fast FLIM images •    Calculate a phasor plot of entire mosaic •    Identify phasor range of objects of interest •    Create segmented image within phasor range selected •    Run precision decay analysis of combined pixels within segmented areas

 

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Figure: Precision FLIM Analysis of the moving leg of a water flee, triple-exponential analysis resolving decay of bound FAD, unbound FAD, and FMN

 

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