The 9th edition of the bh
TCSPC handbook is available for download. On 982 pages, the book
covers the general principles of classic and multi-dimensional
TCSPC techniques, describes detectors and detector principles, and
gives an overview of the performance that can be reached with
various detectors. A large section covers the implementation of
TCSPC in typical experiments of time-resolved spectroscopy. It
describes the measurement of fluorescence and anisotropy decay
curves, multi-spectral lifetime experiments, recording of transient
fluorescence-lifetime phenomena, the measurements of
phosphorescence decay curves, FLIM with various excitation
principles, high-speed and time-series FLIM, Z-stack FLIM,
simultaneous fluorescence and phosphorescence lifetime imaging
(FLIM/PLIM), FLITS, and FLIM with special microscope
configurations. Another part of the book covers TCSPC applications.
It contains chapters on molecular imaging, FRET experiments,
autofluorescence imaging, metabolic imaging, clinical FLIM, NIRS and
fNIRS techniques, correlation experiments, single-molecule
spectroscopy, and FCS. The handbook also contains a large chapter
on bh's new SPCImage NG data analysis software. The book ends with
a list of 1283 references related to TCSPC, most of them being
applications of the bh SPC devices.
For free download please click on the figure below.