자료실

 

자료실

 

 

search

 

 

search

 

 

 

 

 

HOME > 자료실

 

  bh news 2017/11: DCS-120 FLIM system records X-Y mosaics
ㆍ작성자: 전재필 ㆍ작성일: 2017-11-29 (수) 09:50 ㆍ조회: 88

bh News 2017-11:

DCS-120 FLIM system records X-Y mosaics

With software version 9.76, the control of a motorised sample stage has been integrated in the bh SPCM TCSPC/FLIM data acquisition software. In combination with the Mosaic FLIM function of SPCM, the sample stage can be used to record arrays of FLIM images with the bh DCS-120 confocal and multiphoton FLIM systems. The FLIM system scans an image at one position of the sample, then offsets the sample by the size of the scan area, and scans a new image. The process is repeated, combining the images of the individual frames into a single, large x-y-t data set. Images covering an area of several mm diameter can be obtained with high-NA objective lenses. Compared to a single scan with a low-magnification objective lens, a higher collection, a higher 2p excitation efficiency, and a higher optical resolution is obtained.

For details please see application note, please click here to download

  
Left: FLIM mosaic of BPAE cell scample, 248x248 pixels, 256 time channels. Right: Zoom into data shown left